科学研究

Principal Investigators

Magdalena J Koziol Ph.D

E-mail: mjk@cibr.ac.cn

Phone: +44 7724349470

Lab Homepage: https://dnalaboratory.org

Education

Ph.D. in Developmental Biology, University of Cambridge, UK, 2007

B.Sc. in Medical Genetics, University of Leicester, UK, 2000



Professional Experience

06/2019 – present      Principal Investigator, Lab Head of Koziol Laboratory

                                   Chinese Institute for Brain Research, CIBR, Beijing, China

03/2013 ­– 04/2019     Post-Doctoral Scientist with Sir John B Gurdon

                                   Gurdon Institute, University of Cambridge, UK

06/2011 ­– 03/2013     Post-Doctoral Scientist with Antonio Giraldez

                                   Department of Genetics, Yale University, USA

06/2009 – 05/2011     Post-Doctoral Scientist with John Rinn

                                   Broad Institute & Harvard University, USA

07/2007 – 05/2009     Boston Consulting Group

                                   Frankfurt Office, Germany

Research Description

DNA, the ‘building block of life’, form the basis for human health. Since chemical modifications directly alter DNA, discovering and studying such modifications is key in understanding human brain function, in health and disease. DNA modifications affecting one DNA base, dC, have been studied extensively for years, revealing fundamental roles in regulating genes, brain function and in human diseases, such as in cancer. Surprisingly, DNA modifications affecting other DNA building blocks have mostly been ignored. Hence, I have used novel approaches and tried to discover novel DNA modifications in vertebrate genomes. As a proof of principle, I identified a novel DNA modification in vertebrate genomes, called methylated deoxyadenosine (Koziol et al., 2015). This finding opened entirely novel avenues for investigation and pioneered an exciting new area of biology that I we are following up in the Koziol Laboratory. Therefore, in addition to studying this novel DNA modification that is now known to be important for brain function, the Koziol Laboratory is studying and aiming to discover novel DNA and RNA modifications and investigate their role in the brain. Where possible, we explore how our discoveries can be exploited to improve human health

Publications

1.  Pacini C, Bradshaw CR, Garrett NJ, Koziol MJ. Characteristics and homogeneity of N6-methylation in human genomes. submitted, 2019

2. Pacini C, Koziol MJ. Bioinformatics challenges and perspectives when studying the effect of epigenetic modifications on alternative splicing. Philosophical Transactions of the Royal Society B, 2018, doi: 10.1098/rstb.2017.0073

3. Koziol MJ,Bradshaw CR, Allen GE, Costa ASH, Frezza C. Identification of methylated deoxyadenosines by DNA immunoprecipitation. Bio-Protocol, 2016, doi: 10.21769/BioProtoc.1990

4. Koziol MJ, Bradshaw CR, Allen GE, Costa ASH, Frezza C, Gurdon JB. Identification of methylated deoxyadenosines in vertebrates reveals diversity in DNA modifications. Nature Structural & Molecular Biology, 2015, doi: 10.1038/nsmb.3145

5. Koziol MJ, Gurdon JB. TCTP in development and cancer. Biochemistry Research International, 2012, doi: 10.1155/2012/105203

6.  Cabili MN, Trapnell CB, Goff L, Koziol MJ, Tazon-Vega B, Regev A, Rinn LJ. Integrative annotation of human large non-coding RNAs reveals global properties and specific subclasses. Genes and Development, 2011, doi: 10.1101/gad.17446611

7.  Huarte M, Guttman M, Feldser D, Garber M, Koziol MJ, Kenzelmann-Broz, Khalil AM, Zuk O, Amit I, Rabani M, Attardi LD, Regev A, Lander ES, Jacks T, Rinn JL. A large intergenic noncoding RNA induced by p53 mediates global gene repression in the p53 response. Cell, 2010, doi: 10.1016/j.cell.2010.06.040

8.  Guttman M, Garber M, Levin JZ, Donaghey J, Robinson J, Adiconis X, Fan L, Koziol MJ, Gnirke A, Nusbaum C, Rinn JL, Lander ES, Regev A. Ab initio reconstruction of cell type-specific transcriptomes in mouse reveals the conserved multi-exonic structure of lincRNAs. Nature Biotechnology, 2010, doi: 10.1038/nbt.1633

9 Koziol MJ, Rinn JL. RNA traffic control of chromatin complexes. Current Opinions in Genetics & Development, 2010, doi: 10.1016/j.gde.2010.03.003

10.Koziol MJ, Garrett N, Gurdon JB. Tpt1 activates transcription of Oct4 and Nanog in transplanted somatic nuclei. Current Biology, 2007, doi: 10.1016/j.cub.2007.03.062