科学研究

Core Facilities

Fei Zhao Ph.D Director of Vector Engineering Facility

E-mail: zhaofei@cibr.ac.cn

Phone: +86-10-80765986

Lab Homepage:

Education

2010-2012 National Institute of Health, USA (post-doctoral fellow)

2004-2009 Hannover Medical School, Germany (Ph.D student)

2001-2004 Wuhan Institute of Virology, Chinese Academy of Sciences (graduate student)

1997-2001 Huazhong Agriculture University (Bachelor student)

 


Professional Experience

2019-present Chinese Institute for Brain Research, Beijing, China

2012-2018 Wuhan Institute of Virology, Chinese Academy of Sciences (Associate professor)


Research Description

Viral vector and viral tracing tools have been broadly applied in neuroscience researches for gene transfer, neuron labeling, circuits mapping, and function deciphering. The most often used viral vectors and tracing tools include adeno-associated virus (AAV), lentivirus (LV), rabies virus (RABV), vesicular stomatitis virus (VSV), pseudorabies virus (PRV) strain Bartha, herpes simplex virus type 1 (HSV-1) strain H129, etc. AAV and LV are normally applied as vectors to introduce exogenous genes (reporter protein, target genes, or shRNA, etc) into specific neural cells, or used as helper to facilitate the trans-neuronal transmission of mono-synaptic viral tracers (such as RABV or HSV-1-H129) to map direct neural connection. RABV and PRV-Bartha are useful retrograde tracers to reveal input neuronal circuits, and HSV-1-H129 represents a promising anterograde tracer for identification of output network.

The main aim of the Vector Engineering Facility is to provide viral vectors and viral tracing tools to neuroscientists, and also generates and produces customized vectors and tools according to the requirement of the scientists. We will optimize current vectors/tools, improve present production technologies, as well as develop novel systems. Our goal is to provide a powerful gadget pool with high quantity and quality to facilitate neuroscience investigation. 


Publications

1. Sun T, Song Z, Tian Y, Tian W, Zhu C, Ji G, Luo Y, Chen S, Wang L, Mao Y, Xie W, Zhong H, Zhao F, Luo MH, Tao W, Wang H, Li Ji, Li Ju, Zhou J, Wang K*, Zhang Z*. 2019. Basolateral amygdala input to the medial prefrontal cortex controls obsessive-compulsive disorder-like checking behavior. Proc Natl Acad Sci U S A. (in press)

2. Yang B, Liu XJ, Yao Y, Jiang X, Wang XZ, Yang H, Sun JY, Miao Y, Wang W, Huang ZL, Wang Y, Tang Q, Rayner S, Britt WJ, McVoy MA, Luo MH*, Zhao F*. 2018. WDR5 Facilitates Human Cytomegalovirus Replication by Promoting Capsid Nuclear Egress. Journal of virology 92.

3. Zeng WB#, Jiang HF#, Gang YD, Song YG, Shen ZZ, Yang H, Dong X, Tian YL, Ni RJ, Liu Y, Tang N, Li X, Jiang X, Gao D, Androulakis M, He XB, Xia HM, Ming YZ, Lu Y, Zhou JN, Zhang C, Xia XS, Shu Y, Zeng SQ, Xu F*, Zhao F*, Luo MH*. 2017. Anterograde monosynaptic transneuronal tracers derived from herpes simplex virus 1 strain H129. Mol Neurodegener 12:38.

4. Liu XJ, Yang B, Huang SN, Wu CC, Li XJ, Cheng S, Jiang X, Hu F, Ming YZ, Nevels M, Britt WJ, Rayner S, Tang Q, Zeng WB*, Zhao F*, Luo MH*. 2017. Human cytomegalovirus IE1 downregulates Hes1 in neural progenitor cells as a potential E3 ubiquitin ligase. PLoS Pathog 13:e1006542.

5. Yu K, Ahrens S, Zhang X, Schiff H, Ramakrishnan C, Fenno L, Deisseroth K, Zhao F, Luo MH, Gong L, He M, Zhou P, Paninski L, Li B*. 2017. The central amygdala controls learning in the lateral amygdala. Nat Neurosci 20:1680-1685.

6. Wang YY, Wang Y*, Jiang HF, Liu JH, Jia J, Wang K, Zhao F, Luo MH, Luo MM, Wang XM*. 2018. Impaired glutamatergic projection from the motor cortex to the subthalamic nucleus in 6-hydroxydopamine-lesioned hemi-parkinsonian rats. Exp Neurol 300:135-148.

7. Tian Y, Yang C, Cui Y, Su F, Wang Y, Wang Y, Yuan P, Shang S, Li H, Zhao J, Zhu D, Tang S, Cao P, Liu Y, Wang X, Wang L, Zeng W, Jiang H, Zhao F, Luo M, Xiong W, Qiu Z, Li XY, Zhang C*. 2018. An Excitatory Neural Assembly Encodes Short-Term Memory in the Prefrontal Cortex. Cell Rep 22:1734-1744.

8. Zhao F, Jiang HF, Zeng WB, Shu Y, Luo MH*, Duan S. 2017. Anterograde Trans-Synaptic Tagging Mediated by Adeno-Associated Virus. Neurosci Bull 33:348-350.

9. Jiang HF, Wang W, Jiang X, Zeng WB, Shen ZZ, Song YG, Yang H, Liu XJ, Dong X, Zhou J, Sun JY, Yu FL, Guo L, Cheng T, Rayner S, Zhao F, Zhu H*, Luo MH*. 2017. ORF7 of Varicella-Zoster Virus Is Required for Viral Cytoplasmic Envelopment in Differentiated Neuronal Cells. Journal of virology 91.

10. Zhao F#, Shen ZZ#, Liu ZY#, Zeng WB, Cheng S, Ma YP, Rayner S, Yang B, Qiao GH, Jiang HF, Gao S, Zhu H, Xu FQ, Ruan Q*, Luo MH*. 2016. Identification and BAC construction of Han, the first characterized HCMV clinical strain in China. J Med Virol 88:859-870.

11. Duffy A, Zhao F, Haile L, Gamrekelashvili J, Fioravanti S, Ma C, Kapanadze T, Compton K, Figg WD, Greten TF*. 2013. Comparative analysis of monocytic and granulocytic myeloid-derived suppressor cell subsets in patients with gastrointestinal malignancies. Cancer immunology, immunotherapy : CII 62:299-307.

12. Zhao F, Hoechst B, Gamrekelashvili J, Ormandy LA, Voigtlander T, Wedemeyer H, Ylaya K, Wang XW, Hewitt SM, Manns MP, Korangy F*, Greten TF*. 2012. Human CCR4+ CCR6+ Th17 cells suppress autologous CD8+ T cell responses. Journal of immunology 188:6055-6062.

13. Zhao F, Hoechst B, Duffy A, Gamrekelashvili J, Fioravanti S, Manns MP, Greten TF*, Korangy F*. 2012. S100A9 a new marker for monocytic human myeloid-derived suppressor cells. Immunology 136:176-183.

14. Zhao F, Vermeer B, Lehmann U, Kreipe H, Manns MP, Korangy F*, Greten TF*. 2009. Identification of a novel murine pancreatic tumour antigen, which elicits antibody responses in patients with pancreatic carcinoma. Immunology 128:134-140.

15. Zhao F, Obermann S, von Wasielewski R, Haile L, Manns MP, Korangy F*, Greten TF*. 2009. Increase in frequency of myeloid-derived suppressor cells in mice with spontaneous pancreatic carcinoma. Immunology 128:141-149.