2001 B.S. in Microbiology, Huazhong Agriculture University, Wuhan, China
2004 M.S. in Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, China
2009 Ph.D in Immunology, Hannover Medical School, Hannover, Germany
01/2019 - present Director of Vector Core
Chinese Institute for Brain Research (CIBR), Beijing, China
07/2012 - 12/2018 Associate professor
Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
05/2010 - 06/2012 post-doctoral fellow
National Institute of Health, Bethesda, MD, USA
07/2009 – 04/2010 Post-doctoral fellow
Hannover Medical School, Hannover, Germany
Viral vector and viral tracing tools have been broadly applied in neuroscience researches for gene transfer, neuron labeling, circuits mapping, and function deciphering. The most often used viral vectors and tracing tools include adeno-associated virus (AAV), lentivirus (LV), rabies virus (RABV), vesicular stomatitis virus (VSV), pseudorabies virus (PRV) strain Bartha, herpes simplex virus type 1 (HSV-1) strain H129, etc. AAV and LV are normally applied as vectors to introduce exogenous genes (reporter protein, target genes, or shRNA, etc) into specific neural cells, or used as helper to facilitate the trans-neuronal transmission of mono-synaptic viral tracers (such as RABV or HSV-1-H129) to map direct neural connection. RABV and PRV-Bartha are useful retrograde tracers to reveal input neuronal circuits, and HSV-1-H129 represents a promising anterograde tracer for identification of output network.
The main aim of the Vector Engineering Facility is to provide viral vectors and viral tracing tools to neuroscientists, and also generates and produces customized vectors and tools according to the requirement of the scientists. We will optimize current vectors/tools, improve present production technologies, as well as develop novel systems. Our goal is to provide a powerful gadget pool with high quantity and quality to facilitate neuroscience investigation.